Urocortin Polyklonaler Antikörper

Urocortin Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19731-1-AP

Synonyme

UCN, UI, UROC, urocortin

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 19731-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19731-1-AP (Urocortin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19731-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19731-1-AP (Urocortin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 19731-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 19731-1-AP (Urocortin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 19731-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19731-1-AP (Urocortin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, humanes Hodengewebe, humanes Nierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

19731-1-AP bindet in WB, ELISA Urocortin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: urocortin
• Berechnetes Molekulargewicht: 13 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: NM_003353
• Gene symbol: UCN
• Gene ID (NCBI): 7349
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Urocortin is a member of the sauvagine/corticotropin-releasing factor/urotensin I family that includes CRF, urotensin I, sauvagine, urocortin II and urocortin III. Urocortin is a potent anorexigenic peptide that induces fed-like motor activity when administered centrally or peripherally in fasted animals. Urocortin is also a potent and long-lasting hypotensive agent and increases coronary blood flow. Urocortin acts in vitro to stimulate the secretion of adrenocorticotropic hormone (ACTH) and binds with high affinity to CRF receptor types 1, 2-alpha, and 2-beta. Urocortin is synthesized as precursor, which is subsequently processed to the mature biologically active peptide (a 40-amino acid Molecule ). The 22 kDa may be a urocortin 1 precursor protein (PMID: 28887029).