UCHL5 Monoklonaler Antikörper

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• KD/KO Validated

UCHL5 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

2B8G11

Kat-Nr. : 68535-1-Ig

Synonyme

CGI 70, INO80R, Ubiquitin thioesterase L5, UCH L5, UCH37, UCHL5

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Galerie der Validierungsdaten

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  WB analysis of HCT 116 using 68535-1-Ig

  WB result of UCHL5 antibody (68535-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UCHL5 transfected HCT 116 cells.

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  WB analysis using 68535-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68535-1-Ig (UCHL5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, HeLa-Zellen, Jurkat-Zellen, LNCaP-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68535-1-Ig bindet in WB, ELISA UCHL5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: ubiquitin carboxyl-terminal hydrolase L5
• Berechnetes Molekulargewicht: 326 aa, 37 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC025369
• Gene symbol: UCHL5
• Gene ID (NCBI): 51377
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UCHL5 (Ubiquitin carboxyl-terminal hydrolase isozyme L5) is also named as UCH37 and belongs to the peptidase C12 family. It is functionally linked with multiple protein complexes and signal transduction pathways. UCH37's weight average molecular weight increases as a function of its concentration. By size-exclusion chromatography, the apparent molecular weight of UCH37 was 51, 56, 70, and 109 kDa at 0.15, 0.69, 2.4, and 9.5 mg/mL, respectively. Given UCH37's monomeric molecular weight of 37 kDa and its apparent elongated shape, its tendency toward an apparent molecular weight between 40 and 50 kDa at decreasing concentrations is appropriate for UCH37 monomers (PMID:21953935).