UBXD1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

UBXD1 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 14706-1-AP

Synonyme

UBX domain protein 6, UBXD1, UBXDC2, UBXN6

---

Galerie der Validierungsdaten

• 

  WB analysis of PC-3 using 14706-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14706-1-AP (UBXD1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14706-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14706-1-AP (UBXD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14706-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14706-1-AP (UBXD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14706-1-AP (UBXD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14706-1-AP (UBXD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse brain using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14706-1-AP (UBXD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14706-1-AP (UBXD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14706-1-AP

  Immunofluorescent analysis of Hela cells, using UBXN6 antibody 14706-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HeLa using 14706-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14706-1-AP (UBXD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, HeLa-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Hirngewebe, humanes Hodengewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below

Produktinformation

14706-1-AP bindet in WB, IHC, IF, ELISA UBXD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBXD1 fusion protein Ag6394
• Vollständiger Name: UBX domain protein 6
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC008288
• Gene symbol: UBXN6
• Gene ID (NCBI): 80700
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell Chem Biol; Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase.; Authors - Xiaojun Wu ; KD Validated
human	IF	Acta Neuropathol Commun; Proximity proteomics of C9orf72 dipeptide repeat proteins identifies molecular chaperones as modifiers of poly-GA aggregation.; Authors - Feilin Liu
human	WB	J Immunol; UBX Domain Protein 6 Positively Regulates JAK-STAT1/2 Signaling.; Authors - Harshada Ketkar
human	IF	J Cell Sci; The p97-UBXN1 complex regulates aggresome formation.; Authors - Sirisha Mukkavalli

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Tingting (Verified Customer) (01-16-2020)

Very clear bands

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Trophoblast cell, HEK293T