UBXN1 Polyklonaler Antikörper

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• KD/KO Validated

UBXN1 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 16135-1-AP

Synonyme

2B28, LOC51035, SAKS1, SAPK substrate protein 1, UBA/UBX 33.3 kDa protein, UBX domain protein 1, UBXD10, UBXN1

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Galerie der Validierungsdaten

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  WB analysis using 16135-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16135-1-AP (UBXN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16135-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16135-1-AP (UBXN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 16135-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 16135-1-AP (UBXN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16135-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16135-1-AP (UBXN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 16135-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16135-1-AP (UBXN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 16135-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16135-1-AP (UBXN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 16135-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16135-1-AP (UBXN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, humanes Herzgewebe, humanes Hirngewebe, humanes Lebergewebe, Maus-Cerebellum-Gewebe, Mauslebergewebe, Ramos-Zellen, Rattenlebergewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:30000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

16135-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA UBXN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBXN1 fusion protein Ag9146
• Vollständiger Name: UBX domain protein 1
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC000902
• Gene symbol: UBXN1
• Gene ID (NCBI): 51035
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	EMBO J; A weakened interface in the P182L variant of HSP27 associated with severe Charcot-Marie-Tooth neuropathy causes aberrant binding to interacting proteins.; Authors - T Reid Alderson
human	WB	Cancer Lett; The CRISPR/Cas9 system targeting EGFR exon 17 abrogates NF-κB activation via epigenetic modulation of UBXN1 in EGFRwt/vIII glioma cells.; Authors - Kai Huang
human,mouse	WB,IHC	Front Immunol; PTRF/Cavin-1 as a Novel RNA-Binding Protein Expedites the NF-κB/PD-L1 Axis by Stabilizing lncRNA NEAT1, Contributing to Tumorigenesis and Immune Evasion in Glioblastoma.; Authors - Kaikai Yi
human	WB	Sci Rep; PMID: 29769705; Authors - Shotaro Uchida
human	WB,IF	J Cell Sci; The p97-UBXN1 complex regulates aggresome formation.; Authors - Sirisha Mukkavalli ; KO Validated
human	WB,IP,CoIP,IF	Mol Cell Biol; The VCP-UBXN1 complex mediates triage of ubiquitylated cytosolic proteins bound to the BAG6 complex.; Authors - Rakesh Ganji ; KD Validated