UBR2 Polyklonaler Antikörper

UBR2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 18853-1-AP

Synonyme

C6orf133, KIAA0349, N recognin 2, RP3 392M17.3, UBR2

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Galerie der Validierungsdaten

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  WB analysis of BxPC-3 using 18853-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 18853-1-AP (UBR2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 18853-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18853-1-AP (UBR2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 18853-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 18853-1-AP (UBR2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 18853-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18853-1-AP (UBR2 Antibody) at dilution of 1:50(under 10x lens).

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  IHC staining of human pancreas using 18853-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18853-1-AP (UBR2 Antibody) at dilution of 1:50(under 40x lens).

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  IHC staining of human ovary tumor using 18853-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18853-1-AP (UBR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 18853-1-AP

  Immunofluorescent analysis of HepG2 cells, using UBR2 antibody 18853-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of U2OS using 18853-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using UBR2 antibody (18853-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 18853-1-AP

  Immunofluorescent analysis of HepG2 cells, using UBR2 antibody 18853-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, Maus-Cerebellum-Gewebe, Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: U2OS-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

18853-1-AP bindet in WB, IHC, IF, ELISA UBR2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBR2 fusion protein Ag13446
• Vollständiger Name: ubiquitin protein ligase E3 component n-recognin 2
• Berechnetes Molekulargewicht: 439aa,50 kDa; 1755aa,201 kDa
• Beobachtetes Molekulargewicht: 60-66 kDa
• GenBank-Zugangsnummer: BC064512
• Gene symbol: UBR2
• Gene ID (NCBI): 23304
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBR2(Ubiquitin-protein ligase E3-alpha-2) is also named as C6orf133, KIAA0349 and N-recognin-2. It is a component of the N-end rule pathway and recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. It has four isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
mouse	WB	J Biomed Sci; Interruption of the long non-coding RNA HOTAIR signaling axis ameliorates chemotherapy-induced cachexia in bladder cancer; Authors - Che-Yuan Hu