UBQLN2 Polyklonaler Antikörper

UBQLN2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Rezensionen (1)

Kat-Nr. : 23449-1-AP

Synonyme

CHAP1, CHAP1/DSK2, Dsk2, DSK2 homolog, hPLIC 2, HRIHFB2157, LIC 2, N4BP4, PLIC 2, PLIC2, RIHFB2157, ubiquilin 2, Ubiquilin-2, UBQLN2

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Galerie der Validierungsdaten

• 

  WB analysis of rat brain using 23449-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 23449-1-AP (UBQLN2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23449-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23449-1-AP (UBQLN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 23449-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23449-1-AP (UBQLN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 23449-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23449-1-AP (UBQLN2 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 23449-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23449-1-AP (UBQLN2 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 23449-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 23449-1-AP (UBQLN2 Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 23449-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 23449-1-AP (UBQLN2 Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 23449-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UBQLN2 antibody (23449-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, HEK-293-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

23449-1-AP bindet in WB, IP, IHC, IF, ELISA UBQLN2 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBQLN2 fusion protein Ag20149
• Vollständiger Name: ubiquilin 2
• Berechnetes Molekulargewicht: 624 aa, 66 kDa
• Beobachtetes Molekulargewicht: 66-70 kDa
• GenBank-Zugangsnummer: BC069237
• Gene symbol: UBQLN2
• Gene ID (NCBI): 29978
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Brain; Tamoxifen improves muscle structure and function of Bin1- and Dnm2-related centronuclear myopathies; Authors - Charlotte Gineste
		Hum Mol Genet; Conditional depletion of intellectual disability and Parkinsonism candidate gene ATP6AP2 in fly and mouse induces cognitive impairment and neurodegeneration.; Authors - Aline Dubos
mouse	IF	Brain Pathol; Serpin neuropathology in the P497S UBQLN2 mouse model of ALS/FTD.; Authors - Nicole R Higgins
	WB	J Neurochem; Pathogenic mutation of UBQLN2 impairs its interaction with UBXD8 and disrupts endoplasmic reticulum-associated protein degradation.; Authors - Yuxing Xia
human	WB,IF,IP	F1000Res; The identification of high-preforming antibodies for Ubiquilin-2 for use in Western Blot, immunoprecipitation, and immunofluorescence; Authors - Ian McDowell ; KO Validated
	WB	bioRxiv; Short N-terminal disordered regions and the proline-rich domain are major regulators of phase transitions for full-length UBQLN1, UBQLN2 and UBQLN4; Authors - Thuy P Dao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xingyuan (Verified Customer) (06-22-2023)

Antibody quality was good.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T

UBQLN2 Antibody Western Blot validation (1:1000 dilution) in 293T (Cat no:23449-1-AP)