UBL4A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

UBL4A Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Rezensionen (3)

Kat-Nr. : 14253-1-AP

Synonyme

DX254E, DXS254E, G6PD, GDX, ubiquitin like 4A, Ubiquitin like protein 4A, Ubiquitin like protein GDX, UBL4, UBL4A

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 14253-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14253-1-AP (UBL4A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 14253-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 14253-1-AP (UBL4A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14253-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14253-1-AP (UBL4A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 14253-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14253-1-AP (UBL4A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 14253-1-AP

  IP Result of anti-UBL4A (IP:14253-1-AP, 4ug; Detection:14253-1-AP 1:500) with mouse testis tissue lysate 4400ug.

• 

  IHC staining of human ovary tumor using 14253-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14253-1-AP (UBL4A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary tumor using 14253-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14253-1-AP (UBL4A antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 14253-1-AP

  Immunofluorescent analysis of HepG2 cells, using UBL4A antibody 14253-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hirngewebe, HUVEC-Zellen, Maushodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Produktinformation

14253-1-AP bindet in WB, IP, IHC, IF, ELISA UBL4A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBL4A fusion protein Ag5526
• Vollständiger Name: ubiquitin-like 4A
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC043346
• Gene symbol: UBL4A
• Gene ID (NCBI): 8266
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IF	J Immunol; UBL4A Augments Innate Immunity by Promoting the K63-Linked Ubiquitination of TRAF6.; Authors - Shu-Jie Peng ; KD Validated
human	WB	J Virol; SGTA-dependent regulation of Hsc70 promotes cytosol entry of SV40 from the ER.; Authors - Allison Dupzyk
pig	WB	J Nutr; Intermittent Bolus Compared With Continuous Feeding Enhances Insulin and Amino Acid Signaling to Translation Initiation in Skeletal Muscle of Neonatal Pigs.; Authors - Agus Suryawan
human	WB	J Inherit Metab Dis; Mutations in GET4 disrupt the transmembrane domain recognition complex pathway.; Authors - Mitali A Tambe
pig	WB	Pediatr Res; Preterm birth alters the feeding-induced activation of Akt signaling in the muscle of neonatal piglets; Authors - Agus Suryawan
human	WB,IF,IP	J Exp Clin Cancer Res; UBL4A inhibits autophagy-mediated proliferation and metastasis of pancreatic ductal adenocarcinoma via targeting LAMP1.; Authors - Hongze Chen ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

WEI (Verified Customer) (03-08-2022)

Weak specific band around the target size

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Heart

FH

WEI (Verified Customer) (10-21-2020)

This antibody recognizes a sharp, clear band around 16kDa from mouse hearts.

• Applications: Western Blot
• Cell Tissue Type: Mouse Heart

FH

Tom (Verified Customer) (10-09-2020)

This antibody specifically recognizes mouse UBL4A protein. The band was approximately 17kD.

• Applications: Western Blot