UBE4A Polyklonaler Antikörper

UBE4A Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 21548-1-AP

Synonyme

E4, KIAA0126, UBE4A, UBOX2, UFD2

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 21548-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21548-1-AP (UBE4A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21548-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21548-1-AP (UBE4A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 21548-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21548-1-AP (UBE4A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 21548-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21548-1-AP (UBE4A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 21548-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21548-1-AP (UBE4A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 21548-1-AP

  IP Result of anti-UBE4A (IP:21548-1-AP, 4ug; Detection:21548-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, L02-Zellen, Maushirngewebe
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

21548-1-AP bindet in WB, IP, ELISA UBE4A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBE4A fusion protein Ag16211
• Vollständiger Name: ubiquitination factor E4A (UFD2 homolog, yeast)
• Berechnetes Molekulargewicht: 1073 aa, 124 kDa
• Beobachtetes Molekulargewicht: 123 kDa
• GenBank-Zugangsnummer: BC112367
• Gene symbol: UBE4A
• Gene ID (NCBI): 9354
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Mol Cell; RNF126-Mediated Reubiquitination Is Required for Proteasomal Degradation of p97-Extracted Membrane Proteins.; Authors - Xianyan Hu
human	WB	Biochim Biophys Acta Gene Regul Mech; UBE4A catalyzes NRF1 ubiquitination and facilitates DDI2-mediated NRF1 cleavage; Authors - Xianyan Hu ; KD Validated