• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

UBE3A Monoklonaler Antikörper

UBE3A Monoklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

CloneNo.

3D6H9

Kat-Nr. : 60038-1-Ig

Synonyme

ANCR, AS, E6 AP, E6AP, E6AP ubiquitin protein ligase, EPVE6AP, HPVE6A, UBE3A, ubiquitin protein ligase E3A

Galerie der Validierungsdaten

Filter:
  • Western Blot (WB) analysis of HeLa cells using UBE3A Monoclonal antibody (60038-1-Ig)

    WB analysis of HeLa using 60038-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60038-1-Ig (UBE3A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using UBE3A Monoclonal antibody (60038-1-Ig)

    IHC staining of human kidney using 60038-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney using 60038-1-Ig(UBE3A antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using UBE3A Monoclonal antibody (60038-1-Ig)

    IHC staining of human kidney using 60038-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney using 60038-1-Ig(UBE3A antibody) at dilution of 1:50 (under 40x lens).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen
Erfolgreiche Detektion in IHChumanes Nierengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:200-1:1000
Immunhistochemie (IHC)IHC : 1:20-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

60038-1-Ig bindet in WB, IHC,ELISA UBE3A und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen UBE3A fusion protein Ag0346
Vollständiger Name ubiquitin protein ligase E3A
Berechnetes Molekulargewicht 852 aa, 98 kDa
Beobachtetes Molekulargewicht 100 kDa
GenBank-ZugangsnummerBC002582
Gene symbol UBE3A
Gene ID (NCBI) 7337
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Caprylsäure/Ammoniumsulfat-Präzipitation
Lagerungspuffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBE3A(Ubiquitin-protein ligase E3A) is also named as E6AP, EPVE6AP, HPVE6A and belongs to the E3 ubiquitin protein ligase family. It functions as both an E3 ligase in the ubiquitin proteasome pathway and as a transcriptional coactivator. It is also initially identified as a cellular protein that mediates in vitro association of the human papillomavirus E6 protein with p53, leading to the ubiquitin-dependent degradation of p53(PMID:1661671). Defects in UBE3A are a cause of Angelman syndrome (AS)(PMID:10508479 ). It has 3 isoforms produced by alternative splicing.