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UBE2J2 Polyklonaler Antikörper

UBE2J2 Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17713-1-AP

Synonyme

NCUBE 2, NCUBE2, PRO2121, UBE2J2

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of mouse liver tissue using UBE2J2 Polyclonal antibody (17713-1-AP)

    WB analysis of mouse liver using 17713-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17713-1-AP (UBE2J2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBMauslebergewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 3 publications below

Produktinformation

17713-1-AP bindet in WB, ELISA UBE2J2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen UBE2J2 fusion protein Ag12039
Vollständiger Name ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog, yeast)
Berechnetes Molekulargewicht 259 aa, 29 kDa
Beobachtetes Molekulargewicht 29 kDa
GenBank-ZugangsnummerBC094777
Gene symbol UBE2J2
Gene ID (NCBI) 118424
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Protokolle

Produktspezifische Protokolle
WB protocol for UBE2J2 antibody 17713-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

EMBO Rep

MARCH6 and TRC8 facilitate the quality control of cytosolic and tail-anchored proteins.

Authors - Sandra Stefanovic-Barrett
View Article
humanWB

Mol Cell

Ring domains are essential for GATOR2-dependent mTORC1 activation

Authors - Cong Jiang
View Article
humanWB

Dev Cell

Parallel CRISPR-Cas9 screens identify mechanisms of PLIN2 and lipid droplet regulation

Authors - Melissa A Roberts
View Article

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Anna (Verified Customer) (07-17-2019)

Pretty good antibody. Quite a few background bands, but can see specific band depletes with knockout.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HeLa