UBE2D1/2/3/4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

UBE2D1/2/3/4 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 15475-1-AP

Synonyme

HBUCE1, UBCH5D, UBE2D1, UBE2D1/2/3/4, UBE2D2, UBE2D3, UBE2D4, Ubiquitin carrier protein D4, Ubiquitin protein ligase D4

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Galerie der Validierungsdaten

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  WB analysis using 15475-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15475-1-AP (UBE2D4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15475-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15475-1-AP (UBE2D4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15475-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15475-1-AP (UBE2D4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 15475-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15475-1-AP (UBE2D1/2/3/4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15475-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15475-1-AP (UBE2D1/2/3/4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hodengewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

15475-1-AP bindet in WB, IHC, CoIP, ELISA UBE2D1/2/3/4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBE2D1/2/3/4 fusion protein Ag7779
• Vollständiger Name: ubiquitin-conjugating enzyme E2D 4 (putative)
• Berechnetes Molekulargewicht: 17 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC004104
• Gene symbol: UBE2D4
• Gene ID (NCBI): 51619
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ubiquitination is a post-translational modification pathway or is part of the specific protein-degradation pathway by the 26S proteasome. Ubiquitination of a target protein involves multistep enzymatic reaction catalyzed by a cascade of enzymes including Ub-activating enzymes (E1s), Ub-conjugating enzymes (E2s) and Ub ligases (E3s) (PMID: 23542885). UBE2D family, which has 4 members named as UBE2D1/2/3/4, is an E2 ubiquitin-conjugating enzyme family in the ubiquitin-proteasome system. This antibody can recognize all the four members of UBE2D due to the high homology.

Publikationen

Species	Application	Title
human	WB,CoIP	Infect Agent Cancer; Silencing of UBE2D1 inhibited cell migration in gastric cancer, decreasing ubiquitination of SMAD4.; Authors - Honghu Xie ; KD Validated
rat	WB	Environ Toxicol; Blood RNA-sequencing analysis in acrylamide-induced neurotoxicity and depressive symptoms in rats; Authors - Yng-Tay Chen