UBE2C Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

UBE2C Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

CloneNo.

1C12H8

Kat-Nr. : 66087-1-Ig

Synonyme

UBCH10, UBE2C, Ubiquitin carrier protein C, Ubiquitin protein ligase C

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Galerie der Validierungsdaten

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  WB analysis using 66087-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66087-1-Ig (UBE2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 66087-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66087-1-Ig (UBE2C antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66087-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66087-1-Ig (UBE2C antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66087-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66087-1-Ig (UBE2C antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66087-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66087-1-Ig (UBE2C antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66087-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66087-1-Ig (UBE2C antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66087-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66087-1-Ig (UBE2C antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66087-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBE2C antibody (66087-1-Ig, Clone: 1C12H8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HEK-293-Zellen, HepG2-Zellen, NIH/3T3-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

66087-1-Ig bindet in WB, IHC, IF, ELISA UBE2C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: UBE2C fusion protein Ag19235
• Vollständiger Name: ubiquitin-conjugating enzyme E2C
• Berechnetes Molekulargewicht: 179 aa, 20 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC016292
• Gene symbol: UBE2C
• Gene ID (NCBI): 11065
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBE2C(Ubiquitin-conjugating enzyme E2 C) is also known as UBCH10 and belongs to the ubiquitin-conjugating enzyme family. It is highly expressed in a variety of human primary tumors, and it is able to promote cell proliferation and malignant transformation(PMID:18703417). UBE2C, along with the E3 ligase of the anaphasepromoting complex (APC), catalyzes the ubiquitination of mitotic cyclins A and B, as well as securin. UBE2C is essential for cell cycle progression, and mutation of its active site cysteine confers a dominant-negative phenotype(PMID:17217624).

Publikationen

Species	Application	Title
	WB	Elife; Renal medullary carcinomas depend upon SMARCB1 loss and are sensitive to proteasome inhibition.; Authors - Andrew L Hong
human	IHC,IF	Cancer Lett; LINC01194 recruits NUMA1 to promote ubiquitination of RYR2 to enhance malignant progression in triple-negative breast cancer.; Authors - Duanyang Zhai
human	WB	Int J Biol Sci; PRPF6 promotes androgen receptor/androgen receptor-variant 7 actions in castration-resistant prostate cancer cells.; Authors - Wei Liu
human	WB,IHC	FEBS J; Silencing ubiquitin-conjugating enzyme 2C inhibits proliferation and epithelial-mesenchymal transition in pancreatic ductal adenocarcinoma.; Authors - Xianxing Wang ; KD Validated
human	WB	bioRxiv; Shared and Distinct Mechanisms of UBA1 Inactivation Across Different Diseases; Authors - Jason C Collins
human	WB	Cell Death Dis; N-glycosylation of GDF15 abolishes its inhibitory effect on EGFR in AR inhibitor-resistant prostate cancer cells; Authors - Rong Wang