UBE1 Polyklonaler Antikörper

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• KD/KO Validated

UBE1 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 15912-1-AP

Synonyme

A1S9, A1S9T, A1ST, AMCX1, GXP1, Protein A1S9, SMAX2, UBA1, UBA1A, UBE1, UBE1X, Ubiquitin activating enzyme E1

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Galerie der Validierungsdaten

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  WB analysis of HL-60 using 15912-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15912-1-AP (UBE1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15912-1-AP

  WB result of UBE1 antibody (15912-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBE1 transfected HEK-293 cells.

• 

  WB analysis of HL-60 using 15912-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15912-1-AP (UBE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15912-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15912-1-AP (UBE1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 15912-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15912-1-AP (UBE1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 15912-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 15912-1-AP (UBE1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15912-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15912-1-AP (UBE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 15912-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 15912-1-AP (UBE1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 15912-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15912-1-AP (UBE1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15912-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15912-1-AP (UBE1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, HEK-293-Zellen, HeLa-Zellen, Maus-Kolongewebe, Mausmilzgewebe, Rattenmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 3 publications below

Produktinformation

15912-1-AP bindet in WB, IHC, ELISA UBE1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBE1 fusion protein Ag8703
• Vollständiger Name: ubiquitin-like modifier activating enzyme 1
• Berechnetes Molekulargewicht: 1058 aa, 118 kDa
• Beobachtetes Molekulargewicht: 114-118 kDa
• GenBank-Zugangsnummer: BC013041
• Gene symbol: UBA1
• Gene ID (NCBI): 7317
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBE1(Ubiquitin-activating enzyme E1) is also named as A1S9T, UBE1 and belongs to the ubiquitin-activating E1 family. It catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation and initiates the activation and conjugation of ubiquitin-like proteins. Defects in UBE1 are the cause of spinal muscular atrophy X-linked type 2 (SMAX2). UBE1 has two isoforms with the molecular mass of 118 and 114 kDa.

Publikationen

Species	Application	Title
human	WB	Nat Commun; DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation; Authors - Xiaohua Xu
mouse	IHC	ACS Nano; Graphene Oxide Causes Disordered Zonation Due to Differential Intralobular Localization in the Liver.; Authors - Yakun Wu
human		Cancer Biol Med; CSN6 promotes tumorigenesis of gastric cancer by ubiquitin-independent proteasomal degradation of p16INK4a.; Authors - Wenqi Du
human	WB	Am J Cancer Res; The E3 ubiquitin ligase RBCK1 promotes the invasion and metastasis of hepatocellular carcinoma by destroying the PPARγ/PGC1α complex.; Authors - Zheng Xu
human	IHC	Front Oncol; Ubiquitin-Like Modifier Activating Enzyme 1 as a Novel Diagnostic and Prognostic Indicator That Correlates With Ferroptosis and the Malignant Phenotypes of Liver Cancer Cells.; Authors - Yiru Shan
human	WB	J Mol Med (Berl); Hyper-SUMOylation of SMN induced by SENP2 deficiency decreases its stability and leads to spinal muscular atrophy-like pathology.; Authors - Yuhong Zhang