UBASH3B/STS 1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

UBASH3B/STS 1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 19563-1-AP

Synonyme

Cbl interacting protein p70, KIAA1959, p70, STS 1, STS1, UBASH3B, UBASH3B/STS 1

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 19563-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19563-1-AP (UBASH3B/STS 1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19563-1-AP

  WB result of UBASH3B/STS 1 antibody (19563-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBASH3B/STS 1 transfected HEK-293 cells.

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  WB analysis of mouse lung using 19563-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19563-1-AP (UBASH3B/STS 1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 19563-1-AP

  IP Result of anti-UBASH3B/STS 1 (IP:19563-1-AP, 3ug; Detection:19563-1-AP 1:300) with mouse kidney tissue lysate 6000ug.

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  IHC staining of human hepatocirrhosis using 19563-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19563-1-AP (UBASH3B/STS 1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 19563-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19563-1-AP (UBASH3B/STS 1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 19563-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19563-1-AP (UBASH3B/STS 1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 19563-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19563-1-AP (UBASH3B/STS 1 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Mauslungengewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 1 publications below

Produktinformation

19563-1-AP bindet in WB, IP, IHC, IF, ELISA UBASH3B/STS 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ubiquitin associated and SH3 domain containing, B
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 66-70 kDa
• GenBank-Zugangsnummer: NM_032873
• Gene symbol: UBASH3B
• Gene ID (NCBI): 84959
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBASH3B, also named STS1 or TULA-2, is a protein that contains a ubiquitin-associated domain at the N-terminus, a SH3 domain, and a C-terminal domain with similarities to the catalytic motif of phosphoglycerate mutase. TULA-2 is supposed to form homodimer and interact with ubiquitin and mono-ubiquitinated proteins. TULA-2 functions to promote accumulation of activated target receptors, such as T-cell receptors and EGFR on the cell surface.

Publikationen

Species	Application	Title
human	WB	Cell; Oncogenic Mutations Rewire Signaling Pathways by Switching Protein Recruitment to Phosphotyrosine Sites.; Authors - Alicia Lundby
human,mouse	WB	Biochimie; Ubash3b promotes TPA-mediated suppression of leukemogenesis through accelerated downregulation of PKCδ protein.; Authors - Yao Yao ; KD Validated
mouse	WB	Front Immunol; Targeting the Cbl-b-Notch1 axis as a novel immunotherapeutic strategy to boost CD8+ T-cell responses; Authors - Giulia Monticone ; KO Validated
human	WB	Int J Mol Sci; STS1 and STS2 Phosphatase Inhibitor Baicalein Enhances the Expansion of Hematopoietic and Progenitor Stem Cells and Alleviates 5-Fluorouracil-Induced Myelosuppression; Authors - Na Li
human	WB	Transl Cancer Res; Prazosin inhibits the growth and mobility of osteosarcoma cells; Authors - Ming An
human,mouse	WB,IF	Cell Rep; PSGL-1 attenuates early TCR signaling to suppress CD8+ T cell progenitor differentiation and elicit terminal CD8+ T cell exhaustion; Authors - Jennifer L Hope