UBAC2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

UBAC2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, RIP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 25122-1-AP

Synonyme

PHGDHL1, UBA domain containing 2, UBAC2

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 25122-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25122-1-AP (UBAC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 25122-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25122-1-AP (UBAC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 25122-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25122-1-AP (UBAC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 25122-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25122-1-AP (UBAC2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 25122-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25122-1-AP (UBAC2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 25122-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25122-1-AP (UBAC2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 25122-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25122-1-AP (UBAC2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HEK-293 using 25122-1-AP

  Immunofluorescent analysis of HEK-293 cells using 25122-1-AP (UBAC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HEK-293-Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Produktinformation

25122-1-AP bindet in WB, RIP, IHC, IF, ELISA UBAC2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBAC2 fusion protein Ag19142
• Vollständiger Name: UBA domain containing 2
• Berechnetes Molekulargewicht: 344 aa, 39 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC121139
• Gene symbol: UBAC2
• Gene ID (NCBI): 337867
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Extracell Vesicles; A multi-omics approach identifies pancreatic cancer cell extracellular vesicles as mediators of the unfolded protein response in normal pancreatic epithelial cells.; Authors - Charles P Hinzman
human	WB,IHC,IF,RIP	Cell Death Dis; UBAC2 promotes bladder cancer proliferation through BCRC-3/miRNA-182-5p/p27 axis.; Authors - Chaohui Gu ; KD Validated
human	WB	Mol Cell; TMUB1 is an endoplasmic reticulum-resident escortase that promotes the p97-mediated extraction of membrane proteins for degradation; Authors - Linhan Wang