UAP1 Monoklonaler Antikörper

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• KD/KO Validated

UAP1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

2C7B2

Kat-Nr. : 67545-1-Ig

Synonyme

AgX, AGX 2, AGX1, Antigen X, SPAG2, UAP1

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Galerie der Validierungsdaten

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  WB analysis using 67545-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67545-1-Ig (UAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67545-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67545-1-Ig (UAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 67545-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67545-1-Ig (UAP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 67545-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67545-1-Ig (UAP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 67545-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using UAP1 antibody (67545-1-Ig, Clone: 2C7B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, DU 145-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, NCI-H1299-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

67545-1-Ig bindet in WB, IHC, IF, CoIP, ELISA UAP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: UAP1 fusion protein Ag9513
• Vollständiger Name: UDP-N-acteylglucosamine pyrophosphorylase 1
• Berechnetes Molekulargewicht: 505 aa, 57 kDa
• Beobachtetes Molekulargewicht: 57-59 kDa
• GenBank-Zugangsnummer: BC009377
• Gene symbol: UAP1
• Gene ID (NCBI): 6675
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UAP1 can convert UTP and GlcNAc-1-P into UDP-GlcNAc, it can also convert UTP and GalNAc-1-P into UDP-GalNAc. There are three isoforms of UAP1, among which isoform  AGX1  has 2 to 3 times higher activity towards GalNAc-1-P, while isoform  AGX2  has 8 times more activity towards GlcNAc-1-P. It has been reported that UAP1 is specific and highly expressed in prostate cancer. UAP1 can protect cells against inhibitors of N-linked glycosylation. UAP1 may play an important role in maintaining INS responsiveness. 67545-1-Ig detects 57 and 59 kDa isoforms in SDS-PAGE.(PMID:25241896)

Publikationen

Species	Application	Title
human	WB,CoIP	Cell Res; Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P; Authors - Yuan-Li Ai
human	WB	Mol Cell; Metabolic enzyme UAP1 mediates IRF3 pyrophosphorylation to facilitate innate immune response; Authors - Shuai Yang ; KD Validated
human	IHC,WB	Front Immunol; The hexosamine biosynthesis pathway-related gene signature correlates with immune infiltration and predicts prognosis of patients with osteosarcoma; Authors - Zexin Su