U2AF35 Monoklonaler Antikörper

U2AF35 Monoklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG2c

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

2B7F11

Kat-Nr. : 60289-1-Ig

Synonyme

FP793, RN, RNU2AF1, U2AF1, U2AF35, U2AFBP

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 60289-1-Ig

  WB result of U2AF35 antibody (60289-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-U2AF35 transfected HeLa cells.

• 

  WB analysis using 60289-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60289-1-Ig (U2AF35 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 60289-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60289-1-Ig (U2AF35 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 60289-1-Ig

  IP Result of anti-U2AF35 (IP:60289-1-Ig, 3ug; Detection:60289-1-Ig 1:500) with HeLa cells lysate 3000ug.

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  IHC staining of human breast cancer using 60289-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60289-1-Ig (U2AF35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 60289-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60289-1-Ig (U2AF35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 60289-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60289-1-Ig (U2AF35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60289-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60289-1-Ig (U2AF35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 60289-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using U2AF35 antibody (60289-1-Ig, Clone: 2B7F11 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 60289-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using U2AF35 antibody (60289-1-Ig, Clone: 2B7F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of Ramos using 60289-1-Ig

  1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human U2AF35 (60289-1-Ig, Clone:2B7F11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COLO 320-Zellen, HEK-293-Zellen, Raji-Zellen, Ramos-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC: Ramos-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:1000-1:4000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

60289-1-Ig bindet in WB, IP, IHC, IF, FC, ELISA U2AF35 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2c
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: U2AF35 fusion protein Ag0399
• Vollständiger Name: U2 small nuclear RNA auxiliary factor 1
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC001923
• Gene symbol: U2AF1
• Gene ID (NCBI): 7307
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

U2 auxiliary factor (U2AF), comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2 (RNU2) small nuclear RNA auxiliary factor 1 (U2AF1,synonyms: RN, FP793, U2AF35, U2AFBP, RNU2AF1)is the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. The U2AF1 gene is localized to chromosome 21q22.3, which is the critical region for three diseases, progressive myoclonus epilepsy, autoimmune polyglandular disease type 1, and one form of bipolar affective disorder. The calculated molecular weight of U2AF35 is 28 kDa, but the modified U2AF35 protein is about 35-40 kDa.

Publikationen

Species	Application	Title
human	WB	Nat Commun; SPF45/RBM17-dependent, but not U2AF-dependent, splicing in a distinct subset of human short introns.; Authors - Kazuhiro Fukumura
human	WB	Mol Neurodegener; Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing.; Authors - Qingyang Zhang
human	WB	Nucleic Acids Res; Stoichiometries of U2AF35, U2AF65 and U2 snRNP reveal new early spliceosome assembly pathways.; Authors - Li Chen
human	WB	RNA; A model for DHX15 mediated disassembly of A-complex spliceosomes; Authors - Hannah M Maul-Newby