Tubulin Beta Class V Monoklonaler Antikörper

Tubulin Beta Class V Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Hund, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

1D7B9

Kat-Nr. : 66362-1-Ig

Synonyme

HsT1601, TUBB 5, TUBB6, Tubulin beta 6 chain, Tubulin beta class V, tubulin, beta 6

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 66362-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 66362-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66362-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 66362-1-Ig

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 66362-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66362-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66362-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66362-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MDCK using 66362-1-Ig

  Immunofluorescent analysis of (4% PFA ) fixed MDCK cells using 66362-1-Ig(Tubulin beta class V antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hodengewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MDCK-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

66362-1-Ig bindet in WB, IHC, IF, ELISA Tubulin Beta Class V und zeigt Reaktivität mit human, Hund, Maus

• Getestete Reaktivität: human, Hund, Maus
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tubulin, beta 6
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: NM_032525
• Gene symbol: TUBB6
• Gene ID (NCBI): 84617
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tubulin beta class V, encoded by TUBB6, is an isotype of beta tubulin, the major component of microtubule. It is widely expressed in many tissues. In normal tissues, tubulin beta class V is focally expressed in ciliary structure, while in cancer tissues it exhibited a diffuse staining pattern. The alterations of its expression in tumors suggest tubulin beta class V may be used as a biomarker for some solid tumors.

Publikationen

Species	Application	Title
mouse	WB	Mediators Inflamm; LncRNA MDRL Mitigates Atherosclerosis through miR-361/SQSTM1/NLRP3 Signaling; Authors - Ling You
rat	WB	Life Sci; NPY stimulates cholesterol synthesis acutely by activating the SREBP2-HMGCR pathway through the Y1 and Y5 receptors in murine hepatocytes.; Authors - Fu Chen
mouse	WB	Dev Neurobiol; Alpha- and beta-tubulin isotypes are differentially expressed during brain development.; Authors - Torben J Hausrat
rat	IHC	BMC Genomics; Proteomics and transcriptomics profiling reveals distinct aspects of kidney stone related genes in calculi rats; Authors - Wang Zhu