Transgelin-2-specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Transgelin-2-specific Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 15508-1-AP

Synonyme

TAGLN2, Transgelin 2, Transgelin-2-specific

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 15508-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15508-1-AP (Transgelin-2-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15508-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15508-1-AP (Transgelin-2-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 15508-1-AP

  IP Result of anti-Transgelin-2-specific (IP:15508-1-AP, 3ug; Detection:15508-1-AP 1:500) with L02 cells lysate 1000ug.

• 

  IHC staining of human kidney using 15508-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15508-1-AP (Transgelin-2-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human oesophagus cancer using 15508-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15508-1-AP (Transgelin-2-specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 15508-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15508-1-AP (Transgelin-2-specific Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15508-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15508-1-AP (Transgelin-2-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 15508-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 15508-1-AP (Transgelin-2-specific Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 15508-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 15508-1-AP (Transgelin-2-specific Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 15508-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Transgelin-2-specific antibody (15508-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 15508-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug Transgelin-2-specific antibody (15508-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen
• Erfolgreiche IP: L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Ösophaguskarzinomgewebe, humanes Lebergewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

15508-1-AP bindet in WB, IP, IHC, IF, FC, ELISA Transgelin-2-specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: transgelin 2
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: NM_003564
• Gene symbol: TAGLN2
• Gene ID (NCBI): 8407
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody can bind transgelin 2 specifically, but not trangelin 1 or 3.

Publikationen

Species	Application	Title
human	WB	Front Oncol; An Essential NRP1-Mediated Role for Tagln2 in Gastric Cancer Angiogenesis.; Authors - Hongwei Jin ; KD Validated
human	WB, IHC	J Gastroenterol Hepatol; The tumor-suppressive function of miR-1 by targeting LASP1 and TAGLN2 in esophageal squamous cell carcinoma.; Authors - Yan-Yan Du
human	WB	Cancer Cell Int; MiR-613 inhibits the proliferation, migration, and invasion of papillary thyroid carcinoma cells by directly targeting TAGLN2.; Authors - Yonglian Huang
human	WB,IF	PLoS One; Transgelin 2 participates in lovastatin-induced anti-angiogenic effects in endothelial cells through a phosphorylated myosin light chain-related mechanism.; Authors - Xiao Yuan Y ; KD Validated
human	WB,IP	Biochem Biophys Res Commun; Transgelin-2 contributes to proliferation and progression of hepatocellular carcinoma via regulating Annexin A2.; Authors - Jingpei Shi
human	WB	Molecules; MOBT Alleviates Pulmonary Fibrosis through an lncITPF-hnRNP-l-Complex-Mediated Signaling Pathway; Authors - Pan Xu

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