TXNRD2 Polyklonaler Antikörper

TXNRD2 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 16360-1-AP

Synonyme

KIAA1652, Selenoprotein Z, SELZ, thioredoxin reductase 2, Thioredoxin reductase TR3, TR, TR BETA, TR3, TRXR2, TXNRD2

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Galerie der Validierungsdaten

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  WB analysis using 16360-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16360-1-AP (TXNRD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 16360-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16360-1-AP (TXNRD2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 16360-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16360-1-AP (TXNRD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 16360-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16360-1-AP (TXNRD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 16360-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16360-1-AP (TXNRD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, MCF-7-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

16360-1-AP bindet in WB, IHC, IF, ELISA TXNRD2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TXNRD2 fusion protein Ag8367
• Vollständiger Name: thioredoxin reductase 2
• Berechnetes Molekulargewicht: 524 aa, 56 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC007489
• Gene symbol: TXNRD2
• Gene ID (NCBI): 10587
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human mitochondrial thioredoxin reductase (TXNRD2) is a selenocysteine-containing enzyme essential for mitochondrial oxygen radical scavenging. Three mammalian thioredoxin reductases exist; a cytosolic (TXNRD1), a mitochondrial (TXNRD2), and a testis-specific thioredoxin reductase (TXNRD3). (PMID: 21247928, PMID: 26199228)

Publikationen

Species	Application	Title
human	WB	Nat Chem Biol; Ribosome stalling during selenoprotein translation exposes a ferroptosis vulnerability.; Authors - Zhipeng Li
rat	WB,IF	Oxid Med Cell Longev; Paeoniflorin Upregulates Mitochondrial Thioredoxin of Schwann Cells to Improve Diabetic Peripheral Neuropathy Indicated by 4D Label-Free Quantitative Proteomics.; Authors - Xinwei Yang
mouse	WB	Antioxidants (Basel); Selenium Status in Diet Affects Nephrotoxicity Induced by Cisplatin in Mice.; Authors - Shuang Liu
mouse	WB	J Biol Chem; Redox regulation by TXNRD3 during epididymal maturation underlies capacitation-associated mitochondrial activity and sperm motility in mice.; Authors - Huafeng Wang
mouse	WB	Sci Rep; Selenocysteine insertion sequence binding protein 2 (Sbp2) in the sex-specific regulation of selenoprotein gene expression in mouse pancreatic islets.; Authors - B Chellan
mouse	WB,IHC	Photochem Photobiol Sci; Tremella fuciformis polysaccharides alleviates UV-provoked skin cell damage via regulation of thioredoxin interacting protein and thioredoxin reductase 2; Authors - Mengting Lin