TXNDC5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TXNDC5 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 19834-1-AP

Synonyme

EndoPDI, ER protein 46, ERP46, Hcc 2, PDIA15, Thioredoxin like protein p46, TLP46, TXNDC5, UNQ364

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Galerie der Validierungsdaten

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  WB analysis using 19834-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19834-1-AP (TXNDC5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 19834-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19834-1-AP (TXNDC5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 19834-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 19834-1-AP (TXNDC5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 19834-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19834-1-AP (TXNDC5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 19834-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19834-1-AP (TXNDC5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 19834-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19834-1-AP (TXNDC5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 19834-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19834-1-AP (TXNDC5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 19834-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 19834-1-AP (TXNDC5 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 19834-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 19834-1-AP (TXNDC5 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human endometrial cancer using 19834-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19834-1-AP (TXNDC5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 19834-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19834-1-AP (TXNDC5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 19834-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19834-1-AP (TXNDC5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 19834-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19834-1-AP (TXNDC5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 19834-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TXNDC5 antibody (19834-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, COLO 320-Zellen, humanes Plazenta-Gewebe, humanes Skelettmuskelgewebe, Maus-Kolongewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Endometriumkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:10000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

19834-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA TXNDC5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TXNDC5 fusion protein Ag13808
• Vollständiger Name: thioredoxin domain containing 5 (endoplasmic reticulum)
• Berechnetes Molekulargewicht: 432 aa, 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC001199
• Gene symbol: TXNDC5
• Gene ID (NCBI): 81567
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IF,IP	Sci Adv; Targeting mechanosensitive endothelial TXNDC5 to stabilize eNOS and reduce atherosclerosis in vivo.; Authors - Chih-Fan Yeh ; KD Validated
human,mouse	WB,IHC,IF	Nat Commun; Fibroblast-enriched endoplasmic reticulum protein TXNDC5 promotes pulmonary fibrosis by augmenting TGFβ signaling through TGFBR1 stabilization.; Authors - Tzu-Han Lee ; KO Validated
mouse	IHC,IF,WB,CoIP,FC	J Clin Invest; Endoplasmic reticulum protein TXNDC5 promotes renal fibrosis by enforcing TGFβ signaling in kidney fibroblasts.; Authors - Yen-Ting Chen ; KD Validated
mouse	WB,CoIP	Int J Mol Sci; Endoplasmic Reticulum Protein TXNDC5 Interacts with PRDX6 and HSPA9 to Regulate Glutathione Metabolism and Lipid Peroxidation in the Hepatic AML12 Cell Line; Authors - Seyed Hesamoddin Bidooki
mouse	WB	Antioxidants (Basel); Counteracting Cisplatin-Induced Testicular Damages by Natural Polyphenol Constituent Honokiol.; Authors - Tse-En Wang
mouse	WB	Antioxidants (Basel); Squalene Loaded Nanoparticles Effectively Protect Hepatic AML12 Cell Lines against Oxidative and Endoplasmic Reticulum Stress in a TXNDC5-Dependent Way.; Authors - Seyed Hesamoddin Bidooki