TXNDC17 Polyklonaler Antikörper

TXNDC17 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 20811-1-AP

Synonyme

Protein 42 9 9, Thioredoxin like protein 5, TRP14, TXNDC17, TXNL5

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Galerie der Validierungsdaten

• 

  WB analysis of Jurkat using 20811-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20811-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20811-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 20811-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 20811-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 20811-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human uterus using 20811-1-AP

  human uterus tissue were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 20811-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 20811-1-AP

  IP result of anti-TXNDC17 (IP:20811-1-AP, 4ug; Detection:20811-1-AP 1:1000) with HEK-293 cells lysate 2360 ug.

• 

  IF Staining of HeLa using 20811-1-AP

  Immunofluorescent analysis of Hela cells, using TXNDC17 antibody 20811-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HepG2 using 20811-1-AP

  Immunofluorescent analysis of HepG2 cells, using TXNDC17 antibody 20811-1-AP at 1:25 dilution and FITC- labeled donkey anti-Rabbit IgG (green).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, HeLa-Zellen, humanes Nierengewebe, humanes Uterusgewebe, K-562-Zellen, L02-Zellen, Maus-Pankreasgewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

20811-1-AP bindet in WB, IP, IF, ELISA TXNDC17 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TXNDC17 fusion protein Ag14766
• Vollständiger Name: thioredoxin domain containing 17
• Berechnetes Molekulargewicht: 123 aa, 14 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC006405
• Gene symbol: TXNDC17
• Gene ID (NCBI): 84817
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Int J Radiat Biol; Combination of podophyllotoxin and rutin modulate radiation-induced alterations of jejunal proteome in mice.; Authors - Sania Bajaj