TUSC3 Polyklonaler Antikörper

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• KD/KO Validated

TUSC3 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 16039-1-AP

Synonyme

M33, MRT7, N33, OST3A, Protein N33, tumor suppressor candidate 3, TUSC3

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 16039-1-AP

  WB result of TUSC3 antibody (16039-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-TUSC3 transfected HeLa cells.

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  WB analysis using 16039-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16039-1-AP (TUSC3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 16039-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 16039-1-AP (TUSC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Maushirngewebe, Rattenhodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 1 publications below

Produktinformation

16039-1-AP bindet in WB, IHC, ELISA TUSC3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TUSC3 fusion protein Ag8912
• Vollständiger Name: tumor suppressor candidate 3
• Berechnetes Molekulargewicht: 347 aa, 40 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC010370
• Gene symbol: TUSC3
• Gene ID (NCBI): 7991
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TUSC3 (tumor suppressor candidate 3), originally named N33, is a potential tumor supressor gene. Decreased expression of TUSC3 has been found in various cancers, including prostate cancer, pancreas cancer and ovary cancer. TUSC3 also known as OST3A, is identified as a part of the oligosaccharyl-transferase (OST) complex and plays a crucial role in protein N-glycosylation. TUSC3 mutations have been found in families with non-syndromic autosomal recessive mental retardation.

Publikationen

Species	Application	Title
		Nat Commun; miRNA-mediated TUSC3 deficiency enhances UPR and ERAD to promote metastatic potential of NSCLC.; Authors - Young-Jun Jeon
human	WB	J Cell Biol; Oxidoreductase activity is necessary for N-glycosylation of cysteine-proximal acceptor sites in glycoproteins.; Authors - Natalia A Cherepanova ; KD Validated
human	WB	Sci Rep; Mammalian cells lacking either the cotranslational or posttranslocational oligosaccharyltransferase complex display substrate-dependent defects in asparagine linked glycosylation.; Authors - Natalia A Cherepanova ; KD Validated
human	WB	J Biol Chem; ER entry pathway and glycosylation of GPI-anchored proteins are determined by N-terminal signal sequence and C-terminal GPI-attachment sequence; Authors - Tetsuya Hirata
human	IHC	J Transl Med; Downregulation of TUSC3 promotes EMT and hepatocellular carcinoma progression through LIPC/AKT axis; Authors - Ruxia Deng ; KD Validated
human	WB	J Allergy Clin Immunol; Epigenetic activation of the TUSC3 gene as a potential therapy for XMEN disease; Authors - Haodong Ding