TUB Polyklonaler Antikörper

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• KD/KO Validated

TUB Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 17928-1-AP

Synonyme

rd5, TUB, tubby homolog (mouse), Tubby protein homolog

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 17928-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17928-1-AP (TUB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 17928-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 17928-1-AP (TUB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse uterus using 17928-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 17928-1-AP (TUB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 17928-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17928-1-AP (TUB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17928-1-AP

  IP Result of anti-TUB (IP:17928-1-AP, 4ug; Detection:17928-1-AP 1:1000) with mouse brain tissue lysate 3680ug.

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  IHC staining of human brain using 17928-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17928-1-AP (TUB antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 17928-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17928-1-AP (TUB antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maus-Uterusgewebe, Neuro-2a-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

17928-1-AP bindet in WB, IP, IHC, IF, ChIP, ELISA TUB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TUB fusion protein Ag12354
• Vollständiger Name: tubby homolog (mouse)
• Berechnetes Molekulargewicht: 561 aa, 62 kDa
• Beobachtetes Molekulargewicht: 56 kDa, 62-67 kDa
• GenBank-Zugangsnummer: BC075032
• Gene symbol: TUB
• Gene ID (NCBI): 7275
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Mol Cells; Differential Roles of Tubby Family Proteins in Ciliary Formation and Trafficking.; Authors - Julie J Hong
human	ChIP,WB	Cell Rep; NAD+ supplement potentiates tumor-killing function by rescuing defective TUB-mediated NAMPT transcription in tumor-infiltrated T cells.; Authors - Yuetong Wang ; KD Validated
mouse	IF	Front Neurosci; Distribution of ciliary adaptor proteins tubby and TULP3 in the organ of Corti; Authors - Laura A Lindner