TTC19 Polyklonaler Antikörper

TTC19 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 20875-1-AP

Synonyme

2010204O13Rik, TPR repeat protein 19, TTC19

---

Galerie der Validierungsdaten

• 

  WB analysis using 20875-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20875-1-AP (TTC19 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 20875-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20875-1-AP (TTC19 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20875-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20875-1-AP (TTC19 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 20875-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20875-1-AP (TTC19 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 20875-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20875-1-AP (TTC19 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, HeLa-Zellen, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below

Produktinformation

20875-1-AP bindet in WB, IHC, ELISA TTC19 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TTC19 fusion protein Ag14974
• Vollständiger Name: tetratricopeptide repeat domain 19
• Berechnetes Molekulargewicht: 501 aa, 56 kDa
• Beobachtetes Molekulargewicht: 32-35 kDa
• GenBank-Zugangsnummer: BC011698
• Gene symbol: TTC19
• Gene ID (NCBI): 54902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TTC19 is a subunit of mitochondrial respiratory chain complex III and is proposed to preserve the structural and functional integrity of complex III. Loss-of-function mutations in TTC19 have been found in patients with complex III deficiency associated with heterogeneous neurological syndromes. The precursor TTC19 was detected as a 43 kDa cytoplasmic protein, while the mature TTC19 with the mitochondrial targeting sequence cleaved was observed as 35 kDa by SDS-PAGE. (PMID: 21278747)

Publikationen

Species	Application	Title
human	WB,IHC	Int J Biol Sci; XPOT Disruption Suppresses TNBC Growth through Inhibition of Specific tRNA Nuclear Exportation and TTC19 Expression to Induce Cytokinesis Failure; Authors - Huijuan Dai ; KD Validated
human	IHC	Elife; Proteomic characteristics reveal the signatures and the risks of T1 colorectal cancer metastasis to lymph nodes; Authors - Aojia Zhuang