TSPAN9 Polyklonaler Antikörper

TSPAN9 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 21983-1-AP

Synonyme

NET 5, NET5, PP1057, Tetraspan NET 5, tetraspanin 9, Tspan 9, TSPAN9

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Galerie der Validierungsdaten

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  WB analysis of mouse peripheral blood leukocyte using 21983-1-AP

  mouse peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 21983-1-AP (TSPAN9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 21983-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21983-1-AP (TSPAN9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 21983-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 21983-1-AP (TSPAN9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 21983-1-AP

  IP Result of anti-TSPAN9 (IP:21983-1-AP, 4ug; Detection:21983-1-AP 1:500) with mouse spleen tissue lysate 4800ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Leukozyten aus peripherem Blut der Maus, Mausmilzgewebe, Rattenmilzgewebe
• Erfolgreiche IP: Mausmilzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• FC: See 1 publications below

Produktinformation

21983-1-AP bindet in WB, IP, FC, ELISA TSPAN9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TSPAN9 fusion protein Ag17048
• Vollständiger Name: tetraspanin 9
• Berechnetes Molekulargewicht: 239 aa, 27 kDa
• Beobachtetes Molekulargewicht: 29-33 kDa
• GenBank-Zugangsnummer: BC071881
• Gene symbol: TSPAN9
• Gene ID (NCBI): 10867
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TSPAN9 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN9 is relatively highly expressed in the megakaryocyte/platelet lineage and has a role in regulating platelet function in concert with other platelet tetraspanins and their associated proteins. TSPAN9 has been identified as a glycoprotein (PMID: 18795891). This polyclonal antibody detects endogenous TSPAN9 at a band of 29-33 kDa, which is larger than the calculated molecular weight of TSPAN9 (27 kDa), probably due to glycosylation.

Publikationen

Species	Application	Title
human,mouse,rat	WB,FC	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
human	WB	Adv Sci (Weinh); Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia; Authors - Tiemei Li