TSPAN2 Polyklonaler Antikörper

TSPAN2 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 20463-1-AP

Synonyme

6330415F13Rik, NET3, Tetraspan NET 3, tetraspanin 2, TSN2, TSPAN 2, TSPAN2

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Galerie der Validierungsdaten

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  WB analysis using 20463-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20463-1-AP (TSPAN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 20463-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 20463-1-AP (TSPAN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HL-60 using 20463-1-AP

  IP Result of anti-TSPAN2 (IP:20463-1-AP, 4ug; Detection:20463-1-AP 1:500) with HL-60 cells lysate 960ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, K-562-Zellen
• Erfolgreiche IP: HL-60-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:300-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

20463-1-AP bindet in WB, IP, IF, ELISA TSPAN2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TSPAN2 fusion protein Ag14299
• Vollständiger Name: tetraspanin 2
• Berechnetes Molekulargewicht: 221 aa, 24 kDa
• Beobachtetes Molekulargewicht: 30-35 kDa
• GenBank-Zugangsnummer: BC021675
• Gene symbol: TSPAN2
• Gene ID (NCBI): 10100
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TSPAN2 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. It has been reported that TSPAN2 is involved in cell invasion and motility during lung cancer progression (PMID: 24726368). TSPAN2 enhances cell motility and invasiveness by assisting CD44 in scavenging intracellular reactive oxygen species.

Publikationen

Species	Application	Title
human,mouse	WB	Elife; Conservation and divergence of myelin proteome and oligodendrocyte transcriptome profiles between humans and mice.; Authors - Vasiliki-Ilya Gargareta
human	IF	FASEB J; Selective expression of TSPAN2 in vascular smooth muscle is independently regulated by TGF-β1/SMAD and myocardin/serum response factor.; Authors - Jinjing Zhao