TSPAN14 Polyklonaler Antikörper

TSPAN14 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15314-1-AP

Synonyme

DC TM4F2, tetraspanin 14, TM4SF14, Tspan 14, TSPAN14

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Galerie der Validierungsdaten

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  WB analysis of A549 using 15314-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15314-1-AP (TSPAN14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 15314-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15314-1-AP (TSPAN14 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 15314-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15314-1-AP (TSPAN14 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15314-1-AP bindet in WB, ELISA TSPAN14 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TSPAN14 fusion protein Ag7551
• Vollständiger Name: tetraspanin 14
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC002920
• Gene symbol: TSPAN14
• Gene ID (NCBI): 81619
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TSPAN14 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN14 belongs to the TSPANC8 subfamily, which also include TSPAN5, TSPAN10, TSPAN15, TSPAN17, and TSPAN33. It has been reported that TSPANC8 tetraspanins interact with ADAM10 and have a conserved function in the regulation of ADAM10 trafficking and activity, thereby positively regulating Notch receptor activation (PMID: 23091066; 23035126).