TSPAN1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TSPAN1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 16058-1-AP

Synonyme

9030418M05Rik, NET 1, RP11 322N21.1, Tetraspan NET 1, tetraspanin 1, Tetraspanin TM4 C, TSPAN 1, TSPAN1

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Galerie der Validierungsdaten

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  WB analysis using 16058-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16058-1-AP (TSPAN1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of rat colon using 16058-1-AP

  rat colon tissue were subjected to SDS PAGE followed by western blot with 16058-1-AP (TSPAN1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16058-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16058-1-AP (TSPAN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 16058-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16058-1-AP (TSPAN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 16058-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16058-1-AP (TSPAN1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 16058-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16058-1-AP (TSPAN1 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, COLO 320-Zellen, HeLa-Zellen, Ratten-Kolongewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

16058-1-AP bindet in WB, IP, IHC, IF, ELISA TSPAN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TSPAN1 fusion protein Ag8786
• Vollständiger Name: tetraspanin 1
• Berechnetes Molekulargewicht: 241 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26-30 kDa
• GenBank-Zugangsnummer: BC013404
• Gene symbol: TSPAN1
• Gene ID (NCBI): 10103
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TSPAN1 is a membrane protein of the tetraspanin superfamily, which is characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. Altered TSPAN1 expression has been associated with the progression of some neoplasms including hepatocellular carcinoma, colorectal, gastric, and breast cancers (PMID: 20890423; 19437569; 18822690; 20680643).

Publikationen

Species	Application	Title
human	WB,IHC	Autophagy; TSPAN1 promotes autophagy flux and mediates cooperation between WNT-CTNNB1 signaling and autophagy via the MIR454-FAM83A-TSPAN1 axis in pancreatic cancer.; Authors - Cefan Zhou ; KD Validated
rat	WB,IP,IF	Cell Mol Life Sci; Tetraspanin1 promotes NGF signaling by controlling TrkA receptor proteostasis.; Authors - Facundo Ferrero Restelli ; KD Validated