TSG101 Monoklonaler Antikörper

TSG101 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B7G8

Kat-Nr. : CL488-67381

Synonyme

ESCRT I complex subunit TSG101, Exosome marker, TSG10, TSG101, tumor susceptibility gene 101, VPS23

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Galerie der Validierungsdaten

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  IF Staining of human colon cancer using CL488-67381

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488-conjugated TSG101 antibody (CL488-67381, Clone: 2B7G8 ) at dilution of 1:200.

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  IF Staining of human colon cancer using CL488-67381

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488-conjugated TSG101 antibody (CL488-67381, Clone: 2B7G8 ) at dilution of 1:200.

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  FC experiment of THP-1 using CL488-67381

  1X10^6 THP-1 cells were stained with 0.2 ug CoraLite® Plus 488 Anti-Human TSG101 (CL488-67381, Clone: 2B7G8) (red), or 0.2 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Kolonkarzinomgewebe
• Erfolgreiche Detektion in FC: THP-1-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67381 bindet in IF, FC (Intra) TSG101 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TSG101 fusion protein Ag28569
• Vollständiger Name: tumor susceptibility gene 101
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 43-46 kDa
• GenBank-Zugangsnummer: BC002487
• Gene symbol: TSG101
• Gene ID (NCBI): 7251
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.

Verwandte Produkte

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011