TSC22D4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TSC22D4 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 55017-1-AP

Synonyme

THG 1, THG1, TILZ2, Tsc 22 like protein THG 1, TSC22 domain family protein 4, TSC22 domain family, member 4, TSC22D4

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 55017-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55017-1-AP (TSC22D4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55017-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55017-1-AP (TSC22D4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55017-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55017-1-AP (TSC22D4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55017-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55017-1-AP (TSC22D4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55017-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55017-1-AP (TSC22D4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 55017-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 55017-1-AP (TSC22D4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IP: See 1 publications below

Produktinformation

55017-1-AP bindet in WB, IP, IF, ELISA TSC22D4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: TSC22 domain family, member 4
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 41-45 kDa
• GenBank-Zugangsnummer: NM_030935
• Gene symbol: TSC22D4
• Gene ID (NCBI): 81628
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TSC22D4, also named as TILZ2 and THG-1, is a transcriptional repressor. It is a leucine zipper-containing protein that is highly conserved during evolution. TSC22D4 is transcriptionally up-regulated by many different stimuli, including anti-cancer drugs and growth inhibitors. It plays a suppressive role in tumorigenesis. TSC22D4 interacts with TSC22D1 by forming heterodimers(PMID:10488076).This antibody is specific to TSC22D4.

Publikationen

Species	Application	Title
human	WB,IP	J Cell Physiol; The interplay between TGF-β-stimulated TSC22 domain family proteins regulates cell-cycle dynamics in medulloblastoma cells; Authors - Jessica Dragotto ; KD Validated