TSC2 Monoklonaler Antikörper

TSC2 Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A10B2

Kat-Nr. : 68380-1-Ig

Synonyme

FLJ43106, LAM, TSC2, TSC4, Tuberin, tuberous sclerosis 2, Tuberous sclerosis 2 protein

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Galerie der Validierungsdaten

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  WB analysis using 68380-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 68380-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 68380-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 68380-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 68380-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 68380-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TSC2 antibody (68380-1-Ig, Clone: 1A10B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, PC-3-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68380-1-Ig bindet in WB, IF, ELISA TSC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tuberous sclerosis 2
• Berechnetes Molekulargewicht: 1807 aa, 201 kDa
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: BC150300
• Gene symbol: TSC2
• Gene ID (NCBI): 7249
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TSC2, also named Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling.