HTF9C Polyklonaler Antikörper

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• KD/KO Validated

HTF9C Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 16199-1-AP

Synonyme

HTF9C, TRMT2A

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 16199-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16199-1-AP (HTF9C antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16199-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16199-1-AP (HTF9C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16199-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16199-1-AP (HTF9C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16199-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16199-1-AP (HTF9C antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16199-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16199-1-AP (HTF9C antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 16199-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16199-1-AP (HTF9C antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16199-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16199-1-AP (HTF9C antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 16199-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16199-1-AP (HTF9C antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 16199-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16199-1-AP (HTF9C antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HepG2-Zellen, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

16199-1-AP bindet in WB, IHC, ELISA HTF9C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HTF9C fusion protein Ag9112
• Vollständiger Name: TRM2 tRNA methyltransferase 2 homolog A (S. cerevisiae)
• Berechnetes Molekulargewicht: 625 aa, 69 kDa
• Beobachtetes Molekulargewicht: 42-44 kDa, 68-75 kDa
• GenBank-Zugangsnummer: BC013352
• Gene symbol: TRMT2A
• Gene ID (NCBI): 27037
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

tRNA methyltransferase 2 homolog A (TRMT2A), also known as HTF9C is a novel cell cycle regulated protein, as associated with aggressive clinical course. HTF9C harbors a putative RNA recognition motif (RRM) in the N-terminus and the conserved uracil-C5-methyltransferase domain of the TrmA family in the C-terminus. HTF9C antibody can detect two bands about 68 kDa and 40 kDa (PMID: 20307320).

Publikationen

Species	Application	Title
human	WB	Nat Chem Biol; Activity-based RNA-modifying enzyme probing reveals DUS3L-mediated dihydrouridylation.; Authors - Wei Dai ; KD Validated