TRIP6 Monoklonaler Antikörper

TRIP6 Monoklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

5D8C7

Kat-Nr. : 60205-1-Ig

Synonyme

OIP 1, OIP1, Opa interacting protein 1, TR interacting protein 6, TRIP 6, TRIP6, ZRP 1, Zyxin related protein 1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 60205-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60205-1-Ig (TRIP6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 60205-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 60205-1-Ig(TRIP6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 60205-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 60205-1-Ig(TRIP6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 60205-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60205-1-Ig (TRIP6 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 60205-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60205-1-Ig (TRIP6 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 60205-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60205-1-Ig (TRIP6 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60205-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 60205-1-Ig(TRIP6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 60205-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 60205-1-Ig(TRIP6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 60205-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon using 60205-1-Ig(TRIP6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 60205-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon using 60205-1-Ig(TRIP6 antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of HeLa using 60205-1-Ig

  1X10^6 HeLa cells were stained with 0.2ug TRIP6 antibody (60205-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Gliomgewebe, humanes Kolongewebe, humanes Kolonkarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

60205-1-Ig bindet in WB, IHC, FC, ELISA TRIP6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TRIP6 fusion protein Ag15149
• Vollständiger Name: thyroid hormone receptor interactor 6
• Berechnetes Molekulargewicht: 476 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC004249
• Gene symbol: TRIP6
• Gene ID (NCBI): 7205
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIP6 (thyroid receptor-interacting protein 6), also known as ZRP-1 (zyxin-related protein 1), is a member of the zyxin family that has been implicated in actin reorganization and cell motility. Trip6 has been proposed to transport signals from the cell surface to the nucleus.

Publikationen

Species	Application	Title
	WB	Dev Cell; Nuclear Pores Regulate Muscle Development and Maintenance by Assembling a Localized Mef2C Complex.; Authors - Marcela Raices