TRIM9 Monoklonaler Antikörper

TRIM9 Monoklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

CloneNo.

1D2B10

Kat-Nr. : 67268-1-Ig

Synonyme

KIAA0282, RING finger protein 91, RNF91, SPRING, TRIM9, tripartite motif containing 9

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Galerie der Validierungsdaten

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  WB analysis using 67268-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67268-1-Ig (TRIM9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 67268-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67268-1-Ig (TRIM9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 67268-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67268-1-Ig (TRIM9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat cerebellum using 67268-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67268-1-Ig (TRIM9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 67268-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67268-1-Ig (TRIM9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Hirngewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Rattenhirngewebe, Ratten-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67268-1-Ig bindet in WB, IHC,ELISA TRIM9 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TRIM9 fusion protein Ag27856
• Vollständiger Name: tripartite motif-containing 9
• Berechnetes Molekulargewicht: 80 kDa
• Beobachtetes Molekulargewicht: 90 kDa, 79 kDa, 61 kDa
• GenBank-Zugangsnummer: BC013414
• Gene symbol: TRIM9
• Gene ID (NCBI): 114088
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM9(E3 ubiquitin-protein ligase TRIM9) is also named as RNF91 and belongs to the TRIM/RBCC family. TRIM9 protein is a brain-specific E3 ubiquitin ligase. Importantly, TRIM9 is present in LBs found in DLB and PD, suggesting that TRIM9 not only plays roles in the regulation of neuronal functions, but also participates in the formation or breakdown of abnormal inclusions through its ligase activity and besides the striatum and hippocampus, the highest expression of TRIM9 protein is seen in the frontal, temporal and occipital cortices through the western blot (PMID:20085810). It has been reported that TRIM9 is highly expressed in the cerebral cortex in mouse and human brains and that TRIM9 is decreased in damaged brains in patients who have Parkinson disease and dementia with Lewy bodies(PMID:22337885). It has 3 isoforms with molecular mass of 79, 90 and 61 kDa.