TRIM54 Polyklonaler Antikörper

TRIM54 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 21074-1-AP

Synonyme

MURF, MURF 3, MuRF3, RING finger protein 30, RNF30, TRIM54, tripartite motif containing 54

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Galerie der Validierungsdaten

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  WB analysis of mouse heart using 21074-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 21074-1-AP (TRIM54 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 21074-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 21074-1-AP (TRIM54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 21074-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21074-1-AP (TRIM54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 21074-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 21074-1-AP (TRIM54 Antibody) at dilution of 1:50.

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  IHC staining of human heart using 21074-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 21074-1-AP (TRIM54 Antibody) at dilution of 1:50.

• 

  IHC staining of human skeletal muscle using 21074-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 21074-1-AP (TRIM54 Antibody) at dilution of 1:50.

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  IHC staining of human skeletal muscle using 21074-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 21074-1-AP (TRIM54 Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 21074-1-AP

  Immunofluorescent analysis of HepG2 cells, using TRIM54 antibody 21074-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, humanes Herzgewebe, humanes Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

21074-1-AP bindet in WB, IHC, IF, ELISA TRIM54 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM54 fusion protein Ag15229
• Vollständiger Name: tripartite motif-containing 54
• Berechnetes Molekulargewicht: 358 aa, 40 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC141807
• Gene symbol: TRIM54
• Gene ID (NCBI): 57159
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM54(Tripartite motif-containing protein 54) is also names as MURF, MURF3, RNF30. The expression of TRIM54 is required for skeletal myoblast differentiation and myotube fusion. It is expressed in heart and skeletal muscle specifically (PMID:11243782). It has two isoforms with the molecular weight of 40KDa and 45KDa. It can be dimerizated and hetero-dimerizated to form homooligomer and heterooligomer (PMID:15967462).

Publikationen

Species	Application	Title
human	WB	Front Oncol; Targeting TRIM54/Axin1/β-Catenin Axis Prohibits Proliferation and Metastasis in Hepatocellular Carcinoma.; Authors - Jinrong Zhu
human	WB	J Cell Mol Med; NT5DC2 promotes leiomyosarcoma tumour cell growth via stabilizing unpalmitoylated TEAD4 and generating a positive feedback loop.; Authors - Bowen Hu
human	WB	Hepatol Commun; Sorafenib induces ferroptosis by promoting TRIM54-mediated FSP1 ubiquitination and degradation in hepatocellular carcinoma; Authors - Man-Ru Liu