TRIM46 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TRIM46 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 21026-1-AP

Synonyme

Gene Y protein, GENEY, TRIFIC, TRIM46, tripartite motif containing 46

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Galerie der Validierungsdaten

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  WB analysis of mouse lung using 21026-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21026-1-AP (TRIM46 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 21026-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21026-1-AP (TRIM46 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21026-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21026-1-AP (TRIM46 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung using 21026-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 21026-1-AP (TRIM46 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 21026-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 21026-1-AP (TRIM46 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 21026-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 21026-1-AP (TRIM46 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 21026-1-AP

  Immunofluorescent analysis of HepG2 cells, using TRIM46 antibody 21026-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HeLa using 21026-1-AP

  Immunofluorescent analysis of Hela cells, using TRIM46 antibody 21026-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, HeLa-Zellen, Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

21026-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA TRIM46 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM46 fusion protein Ag15290
• Vollständiger Name: tripartite motif-containing 46
• Berechnetes Molekulargewicht: 759 aa, 83 kDa
• Beobachtetes Molekulargewicht: 55 kDa, 62 kDa
• GenBank-Zugangsnummer: BC069416
• Gene symbol: TRIM46
• Gene ID (NCBI): 80128
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IP	Nat Commun; Multilayered regulations of alternative splicing, NMD, and protein stability control temporal induction and tissue-specific expression of TRIM46 during axon formation.; Authors - John K Vuong
mouse	WB,IF	Cell Rep; The Spatiotemporal Construction of the Axon Initial Segment via KIF3/KAP3/TRIM46 Transport under MARK2 Signaling.; Authors - Sotaro Ichinose
human	IHC	Oncol Res; Tripartite Motif-Containing 46 Promotes Viability and Inhibits Apoptosis of Osteosarcoma Cells by Activating NF-B Signaling Through Ubiquitination of PPAR.; Authors - Wenwei Jiang
human	WB	Exp Cell Res; TRIM46 contributes to high glucose-induced ferroptosis and cell growth inhibition in human retinal capillary endothelial cells by facilitating GPX4 ubiquitination.; Authors - Jingfa Zhang ; KD Validated
human	WB,IF	Int J Mol Sci; Mmu-miR-1894-3p Inhibits Cell Proliferation and Migration of Breast Cancer Cells by Targeting Trim46.; Authors - Li Zhang
human	WB,CoIP	Eye Vis (Lond); TRIM46 aggravated high glucose-induced hyper permeability and inflammatory response in human retinal capillary endothelial cells by promoting IκBα ubiquitination; Authors - Hangqi Shen ; KO Validated