TRIM44 Monoklonaler Antikörper

TRIM44 Monoklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IF,ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

1B12H12

Kat-Nr. : 66249-1-Ig

Synonyme

DIPB, HSA249128, MC7, Protein DIPB, TRIM44, tripartite motif containing 44

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Galerie der Validierungsdaten

• 

  WB analysis of MCF-7 using 66249-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66249-1-Ig (TRIM44 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 66249-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66249-1-Ig (TRIM44 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 66249-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed MCF-7 cells using 66249-1-Ig(TRIM44 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, humanes Hodengewebe
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

66249-1-Ig bindet in WB, IF,ELISA TRIM44 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TRIM44 fusion protein Ag18771
• Vollständiger Name: tripartite motif-containing 44
• Berechnetes Molekulargewicht: 344 aa, 38 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC024031
• Gene symbol: TRIM44
• Gene ID (NCBI): 54765
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM44 is one of the family member of TRIM protein, which contains an N-terminal ubiquitin hydrolase-type zinc-finger domain, followed by a coiled-coil domain, a zinc-finger B-box homology domain, and a second coiled-coil domain near the C-terminus. Members of the TRIM protein family are involved in various cellular processes, such as cell proliferation, oncogenesis and antiviral defense.

Publikationen

Species	Application	Title
human	WB	J Immunol; Inducible TAP1 Negatively Regulates the Antiviral Innate Immune Response by Targeting the TAK1 Complex.; Authors - Zhangchuan Xia
human	WB	J Immunol; NAC1 Potentiates Cellular Antiviral Signaling by Bridging MAVS and TBK1.; Authors - Zhangchuan Xia