TRIM41 Polyklonaler Antikörper

TRIM41 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18468-1-AP

Synonyme

RINCK, TRIM41, tripartite motif containing 41

---

Galerie der Validierungsdaten

• 

  WB analysis of BxPC-3 using 18468-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 18468-1-AP (TRIM41 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 18468-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18468-1-AP (TRIM41 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 18468-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18468-1-AP (TRIM41 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 18468-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18468-1-AP (TRIM41 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 18468-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18468-1-AP (TRIM41 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human plasma using 18468-1-AP

  IP Result of anti-TRIM41 (IP:18468-1-AP, 4ug; Detection:18468-1-AP 1:600) with human plasma lysate 4000ug.

• 

  IHC staining of human skeletal muscle using 18468-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 18468-1-AP (TRIM41 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 18468-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 18468-1-AP (TRIM41 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 18468-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18468-1-AP (TRIM41 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 18468-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18468-1-AP (TRIM41 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 18468-1-AP

  Immunofluorescent analysis of HepG2 cells, using TRIM41 antibody 18468-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, humanes Plazenta-Gewebe, Mauslebergewebe
• Erfolgreiche IP: humanes Plasmagewebe
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18468-1-AP bindet in WB, IP, IHC, IF, ELISA TRIM41 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM41 fusion protein Ag13236
• Vollständiger Name: tripartite motif-containing 41
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 70-72 kDa
• GenBank-Zugangsnummer: BC018765
• Gene symbol: TRIM41
• Gene ID (NCBI): 90933
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.