TRIM26 Polyklonaler Antikörper

TRIM26 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 27013-1-AP

Synonyme

Acid finger protein, AFP, RING finger protein 95, RNF95, TRIM26, tripartite motif containing 26, Zinc finger protein 173, ZNF173

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Galerie der Validierungsdaten

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  WB analysis using 27013-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27013-1-AP (TRIM26 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 27013-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27013-1-AP (TRIM26 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 27013-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 27013-1-AP (TRIM26 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 27013-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 27013-1-AP (TRIM26 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 27013-1-AP

  IP Result of anti-TRIM26 (IP:27013-1-AP, 4ug; Detection:27013-1-AP 1:300) with HeLa cells lysate 2400ug.

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  IHC staining of human pancreas cancer using 27013-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27013-1-AP (TRIM26 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 27013-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27013-1-AP (TRIM26 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 27013-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27013-1-AP (TRIM26 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 27013-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27013-1-AP (TRIM26 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27013-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27013-1-AP (TRIM26 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27013-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27013-1-AP (TRIM26 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, HepG2-Zellen, Maushodengewebe, RAW 264.7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

27013-1-AP bindet in WB, IP, IHC, IF, ELISA TRIM26 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM26 fusion protein Ag25546
• Vollständiger Name: tripartite motif-containing 26
• Berechnetes Molekulargewicht: 62 kDa
• Beobachtetes Molekulargewicht: 62 kDa
• GenBank-Zugangsnummer: BC024039
• Gene symbol: TRIM26
• Gene ID (NCBI): 7726
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM26 is a member of the tripartite motif (TRIM) protein family composed of more than 70 members in human. TRIM proteins share a similar characteristic structure, which includes a RING (R) domain, one or two B-boxes (B), and a coiled coil (CC) domain in the N-terminal and a domain in the C-terminal with variable structures. TRIM26 potentiates virus-triggered IRF3, NF-κB activation, IFN-β induction, and cellular antiviral responses. Further investigation suggests that TRIM26 plays an important role in the antiviral innate immune response by bridging TBK1 to NEMO and mediating TBK1 activation. (PMID: 25763818, PMID: 26611359)

Publikationen

Species	Application	Title
human	WB,IHC	Cell Death Dis; The E3 ligase TRIM26 suppresses ferroptosis through catalyzing K63-linked ubiquitination of GPX4 in glioma; Authors - Zhangjie Wang
pig	WB	Virus Res; TRIM26-mediated degradation of nucleocapsid protein limits porcine reproductive and respiratory syndrome virus-2 infection.; Authors - Pandeng Zhao
mouse	WB	Cell Death Dis; USP39 stabilizes β-catenin by deubiquitination and suppressing E3 ligase TRIM26 pre-mRNA maturation to promote HCC progression; Authors - Weiwei Wang
human	WB,IHC,IF,IP	Int J Biol Sci; TRIM26 promotes non-small cell lung cancer survival by inducing PBX1 degradation; Authors - Yuening Sun ; KD Validated
human	WB,IF	FASEB J; TRIM26 restricts Epstein-Barr virus infection in nasopharyngeal epithelial cells through K48-linked ubiquitination of HSP-90β; Authors - Mingjiao Zhang ; KD Validated