TRIM25 Monoklonaler Antikörper

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• KD/KO Validated

TRIM25 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

1H6G1

Kat-Nr. : 67314-1-Ig

Synonyme

EFP, RING finger protein 147, RNF147, TRIM25, tripartite motif containing 25, Z147, Zinc finger protein 147, ZNF147

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Galerie der Validierungsdaten

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  WB analysis using 67314-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67314-1-Ig (TRIM25 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67314-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67314-1-Ig (TRIM25 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67314-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67314-1-Ig (TRIM25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67314-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67314-1-Ig (TRIM25 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67314-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67314-1-Ig (TRIM25 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 67314-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67314-1-Ig (TRIM25 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 67314-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67314-1-Ig (TRIM25 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293T using 67314-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293T cells using TRIM25 antibody (67314-1-Ig, Clone: 1H6G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U2OS-Zellen, COLO 320-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293T-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

67314-1-Ig bindet in WB, IP, IHC, IF, CoIP, ELISA TRIM25 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TRIM25 fusion protein Ag29325
• Vollständiger Name: tripartite motif-containing 25
• Berechnetes Molekulargewicht: 630 aa, 71 kDa
• Beobachtetes Molekulargewicht: 68-71 kDa
• GenBank-Zugangsnummer: BC016924
• Gene symbol: TRIM25
• Gene ID (NCBI): 7706
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM25 (tripartite motif-containing 25) is also named as EFP, Z147, ZNF147, RNF147 and belongs to the zinc finger protein family and tripartite motif (TRIM) family. It induces the Lys63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity (PMID:17392790). It is also essential not only for DDX58 ubiquitination but also for DDX58-mediated interferon-production and antiviral activity in response to RNA virus infection (PMID: 17392790).

Publikationen

Species	Application	Title
human	WB,CoIP	Elife; RTN3 inhibits RIG-I-mediated antiviral responses by impairing TRIM25-mediated K63-linked polyubiquitination.; Authors - Ziwei Yang ; KD Validated
human	WB	Exp Biol Med (Maywood); Tripartite motif-containing 25 facilitates immunosuppression and inhibits apoptosis of glioma via activating NF-κB.; Authors - Mao-Xu Ge ; KD Validated
human	IHC	Cell Death Dis; TRIM25 regulates oxaliplatin resistance in colorectal cancer by promoting EZH2 stability.; Authors - Sha Zhou
human	WB,IP	Oncogene; TRIM25 promotes temozolomide resistance in glioma by regulating oxidative stress and ferroptotic cell death via the ubiquitination of keap1; Authors - Jianwei Wei ; KD Validated