TRIM21 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

TRIM21 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

CloneNo.

1E8B9

Kat-Nr. : 67136-1-Ig

Synonyme

52 kDa Ro protein, RING finger protein 81, RNF81, Ro(SS A), RO52, SS A, SSA, SSA1, TRIM21, tripartite motif containing 21

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Galerie der Validierungsdaten

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  WB analysis using 67136-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67136-1-Ig (TRIM21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 67136-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67136-1-Ig (TRIM21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67136-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67136-1-Ig (TRIM21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 67136-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67136-1-Ig (TRIM21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67136-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67136-1-Ig (TRIM21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67136-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67136-1-Ig (TRIM21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67136-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67136-1-Ig (TRIM21 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 67136-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67136-1-Ig (TRIM21 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 67136-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67136-1-Ig (TRIM21 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67136-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67136-1-Ig (TRIM21 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67136-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRIM21 antibody (67136-1-Ig, Clone: 1E8B9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, HL-60-Zellen, Jurkat-Zellen, NCI-H1299-Zellen, NIH/3T3-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

67136-1-Ig bindet in WB, IP, IHC, IF, CoIP, ELISA TRIM21 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TRIM21 fusion protein Ag28377
• Vollständiger Name: tripartite motif-containing 21
• Berechnetes Molekulargewicht: 475 aa, 54 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC010861
• Gene symbol: TRIM21
• Gene ID (NCBI): 6737
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM21 has multiple N-terminal zinc finger motifs(has E3 ligase activity), a central leucine zipper, and a potential N-glycosylation site. TRIM3 is a E3 ubiquitin-protein ligase. It can form a ubiquitin ligase complex with E2 UBE2D2, which function not only for the uniquitination of USP4 and IKBKB but also for its self-ubiquitination. It has a role in the regulation of the cell cycle progression and could enhance the decapping activity of DCP2. It can exist in all mammalian cells as a ribonucleoprotein particle , which composed of a single polypeptide and 1 of 4 small RNA molecules. TRIM21 exists various isoforms and range molecular weight of isoforms is about 42-45kDa and 49-54 kDa.

Publikationen

Species	Application	Title
human	WB	Autophagy; Stress granule homeostasis is modulated by TRIM21-mediated ubiquitination of G3BP1 and autophagy-dependent elimination of stress granules; Authors - Cuiwei Yang ; KD Validated
human	WB	Int J Mol Sci; TRIM21 Promotes Rabies Virus Production by Degrading IRF7 through Ubiquitination; Authors - Boyue Zhang ; KD Validated
mouse	WB	Int Immunopharmacol; TRIM21 deficiency confers protection from OGD/R-induced oxidative and inflammatory damage in cultured hippocampal neurons through regulation of the Keap1/Nrf2 pathway.; Authors - Yahong Fu ; KD Validated
human	IP,WB	Cell Death Dis; RPRD1A stabilizes NRF2 and aggravates HCC progression through competing with p62 for TRIM21 binding.; Authors - Xiaofan Feng
human	WB	JCI Insight; Alternative exon usage in TRIM21 determines the antigenicity of Ro52/TRIM21 in systemic lupus erythematosus; Authors - Eduardo Gomez-Bañuelos
human	WB,CoIP	J Med Virol; TRIM21 promotes ubiquitination of SARS-CoV-2 nucleocapsid protein to regulate innate immunity; Authors - Shenglan Mao