TRIM2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TRIM2 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 20356-1-AP

Synonyme

KIAA0517, RING finger protein 86, RNF86, TRIM2, tripartite motif containing 2

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Galerie der Validierungsdaten

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  WB analysis using 20356-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20356-1-AP (TRIM2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20356-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20356-1-AP (TRIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 20356-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 20356-1-AP (TRIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 20356-1-AP

  IP Result of anti-TRIM2 (IP:20356-1-AP, 4ug; Detection:20356-1-AP 1:300) with mouse brain tissue lysate 3200ug.

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  IHC staining of human kidney using 20356-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 20356-1-AP (TRIM2 Antibody) at dilution of 1:50(under 40x lens).

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  IF Staining of HeLa using 20356-1-AP

  Immunofluorescent analysis of Hela cells, using TRIM2 antibody 20356-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maus-Dünndarmgewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

20356-1-AP bindet in WB, IP, IHC, IF, ELISA TRIM2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM2 fusion protein Ag14208
• Vollständiger Name: tripartite motif-containing 2
• Berechnetes Molekulargewicht: 744 aa, 82 kDa
• Beobachtetes Molekulargewicht: 70-85 kDa
• GenBank-Zugangsnummer: BC011052
• Gene symbol: TRIM2
• Gene ID (NCBI): 23321
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tripartite motif-containing 2(TRIM2) is an E3 ubiquitin ligase which directs proteasome-mediated degradation of target proteins by the ubiquitination of NEFL and phosphorylation of BCL2L11. This protein contains an N-terminal RING domain, followed by a B-box-2 domain, a coiled-coil region, and 6 C-terminal NHL repeats. TRIM2 involves in the apoptotic response to ischemia via directing degradation of BIM protein, thus has a role in neuroprotection.

Publikationen

Species	Application	Title
human	IP,WB,IF	Nat Commun; Divergent self-association properties of paralogous proteins TRIM2 and TRIM3 regulate their E3 ligase activity; Authors - Diego Esposito
human	WB,IF	J Transl Med; Stress-induced epinephrine promotes epithelial-to-mesenchymal transition and stemness of CRC through the CEBPB/TRIM2/P53 axis.; Authors - Zili Zhou
human	WB	Cancer Cell Int; TRIM2 directly deubiquitinates and stabilizes Snail1 protein, mediating proliferation and metastasis of lung adenocarcinoma.; Authors - Zhaoxian Lin ; KD Validated