TRIM16 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TRIM16 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 24403-1-AP

Synonyme

EBBP, TRIM16, tripartite motif containing 16

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 24403-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24403-1-AP (TRIM16 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24403-1-AP

  IP Result of anti-TRIM16 (IP:24403-1-AP, 4ug; Detection:24403-1-AP 1:300) with HeLa cells lysate 3960ug.

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  IHC staining of human testis using 24403-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24403-1-AP (TRIM16 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 24403-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24403-1-AP (TRIM16 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human small intestine using 24403-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 24403-1-AP (TRIM16 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human small intestine using 24403-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 24403-1-AP (TRIM16 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 24403-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 24403-1-AP (TRIM16 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

24403-1-AP bindet in WB, IP, IHC, IF, ELISA TRIM16 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM16 fusion protein Ag21406
• Vollständiger Name: tripartite motif-containing 16
• Berechnetes Molekulargewicht: 64 kDa
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC015674
• Gene symbol: TRIM16
• Gene ID (NCBI): 10626
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Front Cell Dev Biol; TRIM16 Promotes Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Modulating CHIP-Mediated Degradation of RUNX2.; Authors - Yi Zhao ; KD Validated
human	WB,IHC	J Genet Genomics; RASSF6-TRIM16 axis promotes cell proliferation, migration and invasion in esophageal squamous cell carcinoma.; Authors - Leilei Zheng
human	WB	Exp Cell Res; TRIM16 protects human periodontal ligament stem cells from oxidative stress-induced damage via activation of PICOT.; Authors - Yi Zhao
human	WB,IHC	Exp Cell Res; TRIM16 overexpression inhibits the metastasis of colorectal cancer through mediating Snail degradation.; Authors - Longhui Ruan ; KD Validated
human	WB	Cell Prolif; G3BP1 coordinates lysophagy activity to protect against compression-induced cell ferroptosis during intervertebral disc degeneration; Authors - Shuai Li
human	WB,IF	Mol Carcinog; TRIM16 E121D variant affects the risk and prognosis of hepatocellular carcinoma by modulating the Wnt/β-catenin pathway; Authors - Shanfeng Li ; KD Validated