TRIM14 Polyklonaler Antikörper

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• KD/KO Validated

TRIM14 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 15742-1-AP

Synonyme

KIAA0129, TRIM14, tripartite motif containing 14

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Galerie der Validierungsdaten

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  WB analysis using 15742-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15742-1-AP (TRIM14 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 15742-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15742-1-AP (TRIM14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 15742-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15742-1-AP (TRIM14 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 15742-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15742-1-AP (TRIM14 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 15742-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15742-1-AP (TRIM14 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 15742-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15742-1-AP (TRIM14 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 15742-1-AP

  Immunofluorescent analysis of Hela cells, using TRIM14 antibody 15742-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, HepG2-Zellen, HL-60-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

15742-1-AP bindet in WB, IP, IHC, IF, ELISA TRIM14 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM14 fusion protein Ag8158
• Vollständiger Name: tripartite motif-containing 14
• Berechnetes Molekulargewicht: 442 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC006333
• Gene symbol: TRIM14
• Gene ID (NCBI): 9830
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tripartite motif-containing 14(TRIM14) belongs to the TRIM protein family that contains 3 zinc-binding domains, a RING, a B-box type1 and a B-box type2 and plays key roles in cellular proliferation, differentiation, development, transformation, and immune response mechanisms . TRIM4 inhibits the transcriptional activity of SPI1 in a dose-dependent manner. It may participate in the early stages of differentiation of embryonic stem cells, leading to the formation of embryoid bodies.

Publikationen

Species	Application	Title
human,mouse	WB,IF,IP	Cell Rep; Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells; Authors - Yingqi Liu ; KO Validated
human	WB	Sci Rep; TRIM14 is a Putative Tumor Suppressor and Regulator of Innate Immune Response in Non-Small Cell Lung Cancer.; Authors - Josephine Hai ; KD Validated
human	WB	Sci Rep; TRIM14 regulates cell proliferation and invasion in osteosarcoma via promotion of the AKT signaling pathway.; Authors - Guoxing Xu ; KD Validated
human	WB	Sci Rep; Expression profiling of TRIM protein family in THP1-derived macrophages following TLR stimulation.; Authors - Mei-Xiu Jiang
human	WB	Front Pharmacol; Hypoxia-Induced Upregulation of lncRNA ELFN1-AS1 Promotes Colon Cancer Growth and Metastasis Through Targeting TRIM14 via Sponging miR-191-5p.; Authors - Xu Jing
human	WB	Mol Med; Human mesenchymal stem cells derived exosomes inhibit the growth of acute myeloid leukemia cells via regulating miR-23b-5p/TRIM14 pathway.; Authors - Hui Cheng ; KD Validated