TRAF2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TRAF2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

25

Kat-Nr. : 26846-1-AP

Synonyme

TRAF2, TRAP, TRAP3

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 26846-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26846-1-AP (TRAF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26846-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26846-1-AP (TRAF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 26846-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 26846-1-AP (TRAF2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 26846-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26846-1-AP (TRAF2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 26846-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26846-1-AP (TRAF2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 26846-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26846-1-AP (TRAF2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 26846-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26846-1-AP (TRAF2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 26846-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 26846-1-AP (TRAF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 26846-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using TRAF2 antibody (26846-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HUVEC using 26846-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HUVEC cells using 26846-1-AP (TRAF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, humanes Hodengewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen, HeLa-Zellen, HUVEC-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 23 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

26846-1-AP bindet in WB, IP, IHC, IF, ELISA TRAF2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRAF2 fusion protein Ag25387
• Vollständiger Name: TNF receptor-associated factor 2
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC032410
• Gene symbol: TRAF2
• Gene ID (NCBI): 7186
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Redox Biol; FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response; Authors - Zhe Wang
human	IHC	J Hematol Oncol; Integrated proteogenomic characterization of urothelial carcinoma of the bladder.; Authors - Ning Xu
mouse	WB	J Hazard Mater; Endoplasmic reticulum stress manipulates autophagic response that antagonizes polybrominated diphenyl ethers quinone induced cytotoxicity in microglial BV2 cells.; Authors - Qiushuang Zhu
human	WB	EMBO Rep; Snail enhances arginine synthesis by inhibiting ubiquitination-mediated degradation of ASS1.; Authors - Hao Jia ; KD Validated
human,mouse	WB,IHC,IF	Free Radic Biol Med; TRAF2 inhibits senescence in hepatocellular carcinoma cells via regulating the ROMO1/ NAD+/SIRT3/SOD2 axis; Authors - Jiping Yao ; KD Validated
mouse	IF	J Nanobiotechnology; The ketone body β-hydroxybutyrate alleviates CoCrMo alloy particles induced osteolysis by regulating NLRP3 inflammasome and osteoclast differentiation.; Authors - Yanglin Wu