TPD52L2 Monoklonaler Antikörper

TPD52L2 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B3C6

Kat-Nr. : CL488-68077

Synonyme

D54, DKFZp686A1765, hD54, TPD52L2, TPD54, tumor protein D52 like 2, Tumor protein D54

---

Galerie der Validierungsdaten

• 

  IF Staining of HCT 116 using CL488-68077

  Immunofluorescent analysis of (-20°C Methanol) fixed HCT 116 cells using CoraLite® Plus 488 TPD52L2 antibody (CL488-68077, Clone: 3B3C6 ) at dilution of 1:200.

• 

  FC experiment of HeLa using CL488-68077

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TPD52L2 (CL488-68077, Clone:3B3C6) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HCT 116-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-68077 bindet in IF, FC (Intra) TPD52L2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TPD52L2 fusion protein Ag30863
• Vollständiger Name: tumor protein D52-like 2
• Berechnetes Molekulargewicht: 206 aa, 22 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC006804
• Gene symbol: TPD52L2
• Gene ID (NCBI): 7165
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tumor protein D52-like proteins (TPD52) are small coiled-coil motif bearing proteins that were first identified in breast carcinoma. Three human TPD52 members had been identified, named hD52 (TPD52), hD53 (TPD52L1), and hD54 (TPD52L2). The most important characteristic of the protein family is a highly conserved coiled-coil motif that is required for homo- and heteromeric interaction with other TPD52-like proteins. TPD52 and related proteins have been implicated in cell proliferation, apoptosis, and vesicle trafficking. TPD52L2 has five isoforms produced by alternative splicing, and its multiple sites have been identified to be phosphorylated. Interaction of TPD52L2 with MAL2, a novel member of the MAL proteolipid family, may be required for the role of TPD52L2 in vesicle transport.

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C