TPD52L2 Monoklonaler Antikörper

TPD52L2 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

3B3C6

Kat-Nr. : 68077-1-Ig

Synonyme

D54, DKFZp686A1765, hD54, TPD52L2, TPD54, tumor protein D52 like 2, Tumor protein D54

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Galerie der Validierungsdaten

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  WB analysis using 68077-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68077-1-Ig (TPD52L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of NCI-H1299 using 68077-1-Ig

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 68077-1-Ig (TPD52L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 68077-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 68077-1-Ig (TPD52L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 68077-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 68077-1-Ig (TPD52L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 68077-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 68077-1-Ig (TPD52L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 68077-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68077-1-Ig (TPD52L2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 68077-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68077-1-Ig (TPD52L2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HCT 116 using 68077-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HCT 116 cells using TPD52L2 antibody (68077-1-Ig, Clone: 3B3C6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Calu-3 cells, C6-Zellen, HCT 116-Zellen, MCF-7-Zellen, NCI-H1299-Zellen, Neuro-2a-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HCT 116-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68077-1-Ig bindet in WB, IHC, IF, ELISA TPD52L2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: tumor protein D52-like 2
• Berechnetes Molekulargewicht: 206 aa, 22 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC006804
• Gene symbol: TPD52L2
• Gene ID (NCBI): 7165
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tumor protein D52-like proteins (TPD52) are small coiled-coil motif bearing proteins that were first identified in breast carcinoma. Three human TPD52 members had been identified, named hD52 (TPD52), hD53 (TPD52L1), and hD54 (TPD52L2). The most important characteristic of the protein family is a highly conserved coiled-coil motif that is required for homo- and heteromeric interaction with other TPD52-like proteins. TPD52 and related proteins have been implicated in cell proliferation, apoptosis, and vesicle trafficking. TPD52L2 has five isoforms produced by alternative splicing, and its multiple sites have been identified to be phosphorylated. Interaction of TPD52L2 with MAL2, a novel member of the MAL proteolipid family, may be required for the role of TPD52L2 in vesicle transport.