TP73 Monoklonaler Antikörper

TP73 Monoklonal Antikörper für WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

1D12A12

Kat-Nr. : 66990-1-Ig

Synonyme

p53 like transcription factor, p53 related protein, P73, TP73, tumor protein p73

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Galerie der Validierungsdaten

• 

  WB analysis of MCF-7 using 66990-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66990-1-Ig (TP73 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66990-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66990-1-Ig (TP73 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66990-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66990-1-Ig (TP73 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66990-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66990-1-Ig (TP73 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66990-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66990-1-Ig (TP73 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66990-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66990-1-Ig (TP73 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66990-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66990-1-Ig (TP73 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• RIP: See 1 publications below

Produktinformation

66990-1-Ig bindet in WB, RIP, ELISA TP73 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TP73 fusion protein Ag26793
• Vollständiger Name: tumor protein p73
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: NM_001126240
• Gene symbol: TP73
• Gene ID (NCBI): 7161
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	RIP	Cell Rep; A p53/LINC00324 positive feedback loop suppresses tumor growth by counteracting SET-mediated transcriptional repression; Authors - Ling Zhang
human	WB	Biochem Pharmacol; Palmatine induces G2/M phase arrest and mitochondrial-associated pathway apoptosis in colon cancer cells by targeting AURKA.; Authors - Xiaojiang Liu
human	WB	Carcinogenesis; Construction and identification of lncRNA/circRNA coregulated ceRNA networks in gemcitabine-resistant bladder carcinoma; Authors - Jingjing Pan