TOX4 Polyklonaler Antikörper

TOX4 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27988-1-AP

Synonyme

C14orf92, KIAA0737, LCP1, MIG7, TOX4

---

Galerie der Validierungsdaten

• 

  WB analysis using 27988-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27988-1-AP (TOX4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat bladder using 27988-1-AP

  Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 27988-1-AP (TOX4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat bladder using 27988-1-AP

  Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 27988-1-AP (TOX4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 27988-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27988-1-AP (TOX4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 27988-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27988-1-AP (TOX4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse bladder using 27988-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 27988-1-AP (TOX4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse bladder using 27988-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 27988-1-AP (TOX4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, K-562-Zellen, MCF-7-Zellen, PC-3-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: Ratten-Blasengewebe, Maus-Blasengewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

27988-1-AP bindet in WB, IHC, ELISA TOX4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TOX4 fusion protein Ag27638
• Vollständiger Name: TOX high mobility group box family member 4
• Berechnetes Molekulargewicht: 621 aa, 66 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC013689
• Gene symbol: TOX4
• Gene ID (NCBI): 9878
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TOX (Thymocyte selection-associated HMG bOX) genes represent a novel gene family and encode a novel nuclear DNA binding protein belonging to a large superfamily of HMG (high mobility group)-box family. TOX proteins consist a small subfamily of proteins, including TOX1, TOX2, TOX3, and TOX4. The TOX subfamily members are highly conserved among vertebrates. TOX4 was recently found to be one of the subunits of the PP1C, which consists of three regulatory proteins, PNUTS, TOX4, and WDR82, and one of the PP1 phosphatases, PP1α, β, andγ25. Tox4 can modulate cell fate by reprogramming from a somatic state into a pluripotent and neuronal fate (PMID:31519808). Despite a predicted molecular mass of TOX4 protein of 66 kDa, the detection band of western blot analysis in many literatures is 100 kDa (PMID:37286708;35365735). This antibody was also detected at around 100 kda.